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主营类目：抗体，ELISA试剂盒，蛋白质，试剂类，多肽，二抗等
℡ 4000-520-616
℡ 4000-520-616
Antibodies-Online（抗体在线）/AIF1 antibody (Allograft Inflammatory Factor 1) (C-Term)/100 μL/ABIN2857032
产品编号：ABIN2857032
市  场 价：¥9323.60
场      地：美国(厂家直采)
产品分类： 抗体类>一抗>标签抗体>
联系QQ：1570468124
电话号码：4000-520-616
邮      箱： info@ebiomall.com
美  元  价：$466.18
品      牌： Antibodies-Online
公      司：Antibodies-Online,inc.
公司分类：
Antibodies-Online（抗体在线）/AIF1 antibody (Allograft Inflammatory Factor 1) (C-Term)/100 μL/ABIN2857032
商品介绍
Antigen
  • AIF-1
  • IBA1
  • IRT-1
  • IRT1
  • AI607846
  • D17H6S50E
  • G1
  • Iba1
  • Bart1
  • iba1
  • mrf-1
  • fa04b11
  • wu:fa04b11
  • AIF
  • AIF1
  • aif1
  • allograftinflammatoryfactor1
  • AIF1
  • Aif1
  • aif1
Alternatives
anti-HumanAIF1antibodyforImmunohistochemistry(Paraffin-embeddedSections)
51anti-HumanAIF1antibodyforImmunohistochemistry(Paraffin-embeddedSections)
30anti-Rat(Rattus)AIF1antibodyforImmunohistochemistry(Paraffin-embeddedSections)
52anti-Mouse(Murine)AIF1antibodyforImmunohistochemistry
49anti-Mouse(Murine)AIF1antibodyforELISA
79anti-Mouse(Murine)AIF1antibodyforWesternBlotting
50anti-Rat(Rattus)AIF1antibodyforImmunohistochemistry
39anti-Rat(Rattus)AIF1antibodyforELISA
70anti-Rat(Rattus)AIF1antibodyforWesternBlotting
119anti-HumanAIF1antibodyforImmunohistochemistry
116anti-HumanAIF1antibodyforELISA
171anti-HumanAIF1antibodyforWesternBlotting
12anti-HumanAIF1antibodyforImmunocytochemistry
15anti-HumanAIF1antibodyforImmunofluorescence
2anti-HumanAIF1antibodyforImmunohistochemistry(FreeFloating)
10anti-HumanAIF1antibodyforImmunohistochemistry(FrozenSections)
2anti-HumanAIF1antibodyforImmunohistochemistry(WholeMount)
7anti-Mouse(Murine)AIF1antibodyforImmunocytochemistry
11anti-Mouse(Murine)AIF1antibodyforImmunofluorescence
2anti-Mouse(Murine)AIF1antibodyforImmunohistochemistry(FreeFloating)
8anti-Mouse(Murine)AIF1antibodyforImmunohistochemistry(FrozenSections)
23anti-Mouse(Murine)AIF1antibodyforImmunohistochemistry(Paraffin-embeddedSections)
2anti-Mouse(Murine)AIF1antibodyforImmunohistochemistry(WholeMount)
8anti-Rat(Rattus)AIF1antibodyforImmunocytochemistry
11anti-Rat(Rattus)AIF1antibodyforImmunofluorescence
2anti-Rat(Rattus)AIF1antibodyforImmunohistochemistry(FreeFloating)
9anti-Rat(Rattus)AIF1antibodyforImmunohistochemistry(FrozenSections)
2anti-Rat(Rattus)AIF1antibodyforImmunohistochemistry(WholeMount)
6anti-Dog(Canine)AIF1antibodyforImmunohistochemistry(Paraffin-embeddedSections)
6anti-Dog(Canine)AIF1antibodyforELISA
9anti-Dog(Canine)AIF1antibodyforWesternBlotting
2anti-Horse(Equine)AIF1antibodyforImmunohistochemistry(Paraffin-embeddedSections)
2anti-Horse(Equine)AIF1antibodyforELISA
2anti-Horse(Equine)AIF1antibodyforWesternBlotting
6anti-Pig(Porcine)AIF1antibodyforImmunohistochemistry(Paraffin-embeddedSections)
8anti-Pig(Porcine)AIF1antibodyforELISA
9anti-Pig(Porcine)AIF1antibodyforWesternBlotting
1anti-HamsterAIF1antibodyforImmunohistochemistry(Paraffin-embeddedSections)
1anti-HamsterAIF1antibodyforELISA
1anti-HamsterAIF1antibodyforWesternBlotting
1anti-MonkeyAIF1antibodyforImmunohistochemistry(Paraffin-embeddedSections)
1anti-MonkeyAIF1antibodyforELISA
1anti-MonkeyAIF1antibodyforWesternBlotting
1anti-Dog(Canine)AIF1antibodyforEnzymeImmunoassay
4anti-HumanAIF1antibodyforEnzymeImmunoassay
3anti-Mouse(Murine)AIF1antibodyforEnzymeImmunoassay
1anti-Pig(Porcine)AIF1antibodyforEnzymeImmunoassay
2anti-Rat(Rattus)AIF1antibodyforEnzymeImmunoassay
4anti-HumanAIF1antibodyforFlowCytometry
4anti-Rat(Rattus)AIF1antibodyforFlowCytometry
13anti-HumanAIF1antibodyforImmunofluorescence(Paraffin-embeddedSections)
13anti-Mouse(Murine)AIF1antibodyforImmunofluorescence(Paraffin-embeddedSections)
13anti-Rat(Rattus)AIF1antibodyforImmunofluorescence(Paraffin-embeddedSections)
6anti-Mouse(Murine)AIF1antibodyforImmunoprecipitation
11anti-HumanAIF1antibodyforImmunoprecipitation
7anti-Rat(Rattus)AIF1antibodyforImmunoprecipitation
2anti-Dog(Canine)AIF1antibodyforImmunohistochemistry
2anti-GuineaPigAIF1antibodyforImmunohistochemistry
2anti-GuineaPigAIF1antibodyforWesternBlotting
3anti-Pig(Porcine)AIF1antibodyforImmunohistochemistry
1anti-HumanAIF1antibodyforImmunohistochemistry(Formalin-fixedParaffin-embeddedSections)
2anti-HumanAIF1antibodyforImmunoassay
1anti-HumanAIF1antibodyforImmunochromatography
1anti-Mouse(Murine)AIF1antibodyforImmunochromatography
3anti-Mouse(Murine)AIF1antibodyforFlowCytometry
1anti-GuineaPigAIF1antibodyforImmunofluorescence(fixedcells)
11anti-HumanAIF1antibodyforImmunofluorescence(fixedcells)
4anti-Mouse(Murine)AIF1antibodyforImmunofluorescence(fixedcells)
6anti-Rat(Rattus)AIF1antibodyforImmunofluorescence(fixedcells)
1anti-Rat(Rattus)AIF1antibodyforImmunoassay
1anti-GuineaPigAIF1antibodyforImmunocytochemistry
1anti-GuineaPigAIF1antibodyforImmunoprecipitation
1anti-Horse(Equine)AIF1antibodyforImmunohistochemistry
1anti-HamsterAIF1antibodyforImmunohistochemistry
1anti-MonkeyAIF1antibodyforImmunohistochemistry
Epitope
C-Term
Reactivity
Human,Mouse(Murine),Rat(Rattus)
Host
Rabbit
Clonality
Conjugate
ThisAIF1antibodyisun-conjugated
Application
Immunohistochemistry(FrozenSections)(IHC(fro)),Immunohistochemistry(Paraffin-embeddedSections)(IHC(p)),Immunohistochemistry(IHC),WesternBlotting(WB)
Options
  • Successfullyvalidated(Immunofluorescence)
  • byCentred"ImmunologiedeMarseille-Luminy
  • No.#101924
  • Date10/19/2017
AntigenAIF1
LotNumber39476
MethodvalidatedImmunofluorescence
PositiveControlPFAfixedtissuesectionbrainofCX3CR1GFPmouse.StainingwithIba1(Wako,019-19741)andDonkeyanti-rabbitFab’2AlexaFluor647
NotesPassed.ImmunostainingofmousebrainsampleswithABIN2857032showsspecificallytheexpectedstainingpattern.
PrimaryAntibodyABIN2857032
SecondaryAntibodydonkeyFab’2anti-rabbitA647conjugatedantibody(JacksonImmunoresearch,711-606-152,lot128806)
Protocol
  • HarvestBrainfrom13weeksoldmicein1xDulbecco’sphosphatebufferedsaline(DPBS)(GibcoLifeTechnologies,14200-067).
  • Fixmousebraininantigenfix(Diapath,P0014)for2hatRT.
  • Washtissuein0.1MpH7.4phosphatebufferatfor1hat4°C.
  • Dehydratetissuein30%sucrosesolutionONat4°C.
  • SnapfreezetissueinTissueFreezingMedium(ElectroMicroscopyScience,72592-C)at-80°C.
  • Cutblocksinto20µmsectionsusingacryostat(Leica,CM3050S).
  • Transfersectionstoaslide.
  • CreateahydrophobicbarrierontheslidearoundsectionswithDakopen(Dako,S2002,lot00081640).
  • Placeslideinahumidifiedchamberandrehydratesectionsin0.1MTrisHClpH7.4for10minatRT.
  • Gentlyremovebufferbytappingslide.
  • Permeabilizetissuein0.1MTrisHClpH7.4containing2%TritonX-100(Sigma,batch015K0039)and0.5%BSA,for20minatRT.
  • IncubatesectionswithprimaryAIF1(c-term)(Antibodies-Online,ABIN2857032,Lot39476)diluted1:500in0.1MTrisHClpH7.4containing2%TritonX-100(Sigma,batch015K0039)and0.5%BSAfor5hatRT.Incubatethenegativecontrlssectionswithdilutedrabbitserum.
  • Washslides1x5minin0.1MTrisHClpH7.4.
  • IncubatesectionswithsecondarydonkeyFab’2anti-rabbitA647conjugatedantibody(JacksonImmunoresearch,711-606-152,lot128806)diluted1:500in0.1MTrisHClpH7.4containing2%TritonX-100and0.5%BSAONatRT.
  • Washslides1x5minin0.1MTrisHClpH7.4.
  • Addapproximately10μlofSlowfade®Goldantifadereagent(Lifetechnologies,536937,lot1226836)foreachsectionandmountcoverslip.
  • ImageacquisitiononaLSM880(Zeiss),20xmagnification,1000resolution.
ExperimentalNotesMicroglialcellsinthebrainoftheCX3CR1GFPmouseexpressingGFPareknowntoexpressAif-1.ImmunostainingofthePFAfixedtissuesectionswithABIN2857032showedtheexpectedco-localizationoftheGFPandAif-1signals.
ValidationImages
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032)Colocalization(C)ofthesignalsinPFAfixedtissuesectionsofCX3CR1GFPmousebr...
AlternativeNameIba1(AIF1AntibodyAbstract)BackgroundThisgeneisinducedbycytokinesandinterferon.Itsproteinproductisthoughttobeinvolvedinnegativeregulationofgrowthofvascularsmoothmusclecells,whichcontributestotheanti-inflammatoryresponsetovesselwalltrauma.Threetranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.MolecularWeight17kDaGeneID199PathwaysSmoothMuscleCellMigration
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  • anti-AlkalinePhosphatase,Placental-Like2Antibodies
  • anti-AlkalinePhosphatase,PlacentalAntibodies
  • anti-AlkalinePhosphatase,Liver/bone/kidneyAntibodies
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  • anti-alpha1Microglobulin/bikuninAntibodies
  • anti-alpha1,4-GalactosyltransferaseAntibodies
  • anti-alphaHemoglobinStabilizingProteinAntibodies
  • anti-alp
品牌介绍

Antibodies online是抗体、ELISA试剂盒与其他相关试剂的在线营销商，提供来自250家著名供应商的1906775种研究试剂，涵盖广泛的研究应用。其中包括1391351种抗体，170000种ELISA试剂盒，197779种试剂盒，28984种二抗以及179554种蛋白质。

 


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