Antibodies-Online/GFP-Trap® A/ABIN509399/200 tests,Antibodies-Online,,Antibodies-Online,抗体在线,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Antibodies-Online/GFP-Trap® A/ABIN509399/200 tests

产品编号：ABIN509399

市场价：¥74812.60

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>糖类>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$3740.63

品牌： Antibodies-Online

公司：Antibodies-Online,inc.

公司分类：

商品介绍

Antigen

greenfluorescentprotein
gfp

Reactivity

Aequoreavictoria

Host

Camelidae

AntibodyType

RecombinantAntibody

Conjugate

AgaroseBeads

Application

AffinityMeasurement(AM),ChromatinImmunoprecipitation(ChIP),EnzymeActivityAssay(EAA),Immunoprecipitation(IP),MassSpectrometry(MS),ProteinComplexImmunoprecipitation(Co-IP),Pull-DownAssay(Pull-Down),Purification(Purif)

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Supplier

SupplierProductNo.

Productdetails ReferencesImagesHandlingApplicationDetailsTargetdetailsbacktotop
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Purpose	GFP-Trap®isahighqualityGFP-bindingproteincoupledtoamonovalentmatrix(agarosebeads)forbiochemicalanalysisofGFPfusionproteinsandtheirinteractingpartners.
Brand	GFP-Trap®
SampleType	CellExtracts
Specificity	Bindingcapacity:10µLGFP-Trap®_Aslurrybinds2.5-3µgofGFP
Cross-Reactivity(Details)	GFP-Trap®specificallybindstoeGFP,wtGFP,GFPS65T,TagGFP,eYFP,YFP,Venus,Citrin,CFP.NobindingtoproteinsderivedfromDsRed,allRFPsandTurboGFPcanbedetected.
Characteristics	Antibodies-extremelypowerfultoolsinbiomedicalresearch-arelargecomplexmolecules(~150kDa)consistingoftwoheavyandtwolightchains.Duetotheircomplexstructure,theuseofantibodiesisoftenlimitedandhinderedbybatch-to-batchvariations. Camelidae(camels,dromedaries,llamasandalpacas)possessfunctionalantibodiesdevoidoflightchains,so-calledheavychainantibodies(hcAbs).hcAbsrecognizeandbindtheirantigensviaasinglevariabledomain(VHH).TheseVHHdomainsarethesmallestintactantigenbindingfragments(~13kDa). Nano-Trapsarebasedonsingledomainantibodyfragments(VHHs)derivedfromalpaca.
Components	GFP-Trap®coupledtoagarosebeads
Materialnotincluded	Lysisbuffer(CoIP),10xRIPAbuffer,Dilutionbuffer,Washbuffer,Elutionbuffer

Targetdetails ReferencesImagesHandlingApplicationDetailsbacktotop
Targetdetails ShowHide
AlternativeName	GFP
Background	Thegreenfluorescentprotein(GFP)andvariantsthereofarewidelyusedtostudythesubcellularlocalizationanddynamicsofproteins.GFPfusionproteinscanbeexpressedindifferentcelltypesatdifferentexpressionlevelsbytransientorstabletransfection.Transientexpressionmayprovidequickinformativeresults,however,inmanycasesitisnecessarytogeneratestablecelllinesthatexpresstheGFPfusionproteinofinterestatalevelsimilartotheoneoftheendogenousprotein.QuantificationofGFPfusionproteinsincellscanbetrickysinceexistingmethods,likefluorescencemicroscopyorWesternBlotting,areoftenshowsinsufficientsignaltonoiseratiosorhighsignalvariABIlities.
ResearchArea	Tags/Labels

ApplicationDetails ReferencesImagesHandlingTargetdetailsbacktotop
ApplicationDetails ShowHide
ApplicationNotes	Greenfluorescentproteins(GFP)andvariantsthereofarewidelyusedtostudyproteinlocalizationanddynamics.ForbiochemicalanalysesincludingmassspectroscopyandenzymeactivitymeasurementstheseGFP-fusionproteinsandtheirinteractingfactorscanbeisolatedfastandefficiently(onestep)viaImmunoprecipitationusingtheGFP-Trap®.TheGFP-Trap®_AenablespurificationofanyproteinofinterestfusedtoGFP.
Comment	Beadsize~90µm
AssayTime	1.5h
Protocol	RobustandversatiletoolforbiochemicalanalysesofGFP-fusionproteins Shortincubationtimes(5-30min) Quantitativeisolationoffusionproteinsandtransientlyboundfactorsfromcellextractsororganelles Lowunspecificbinding Nocontaminatingheavyandlightchainsofconventionalantibodies ApplicableinChromatinImmunoprecipitation(ChIP)
ReagentPreparation	Suggestedbuffercomposition Lysisbuffer(CoIP):10mMTris/ClpH7.5,150mMNaCl,0.5mMEDTA,0.5%NP-40 10xRIPAbuffer:10mMTris/ClpH7.5,150mMNaCl,5mMEDTA,0.1%SDS,1%TritonX-100,1%Deoxycholate Dilutionbuffer:10mMTris/ClpH7.5,150mMNaCl,0.5mMEDTA Washbuffer:10mMTris/ClpH7.5,150mMNaCl,0.5mMEDTA Elutionbuffer:200mMglycinepH2.5
AssayProcedure	Beforeyoustart:Add1mlPBStoyourcellsandscrapethemoffthepetridish.Transfertoprecooledtube,spin3minat500xganddiscardsupernatant.WashcellpellettwicewithicecoldPBS,brieflyresUSPendingthecells. 1.Foroneimmunoprecipitationreactionresuspendcellpellet(~10^7mammaliancells)in200µLlysisbufferbypipetting(orusingasyringe). optional:add1mMPMSFandProteaseinhibitorcocktail(notincluded)tolysisbuffer optionalfornuclear/chromatinproteins:add1mg/mlDNaseand2.5mMMgCl2(notincluded)tolysisbuffer 2.Placethetubeonicefor30minwithextensivelypipettingevery10min. 3.Spincelllysateat20.000xgfor5-10minutesat4°C. 4.Transfersupernatanttoapre-cooledtube.Adjustvolumewithdilutionbufferto500µL–1000µL.Discardpellet. optional:add1mMPMSFandProteaseinhibitorcocktail(notincluded)todilutionbuffer note:thecelllysatecanbefrozenatthispointforlong-termstorageat-80°CForimmunoblotanalysisdilute50µLcelllysatewith50µL2xSDS-samplebuffer(àrefertoasinput). 5.EquilibrateGFP-Trap®_Abeadsindilutionbuffer.Resuspend20-30µLbeadslurryin500µLicecolddilutionbufferandspindownat2.500xgfor2minutesat4°C.Discardsupernatantandwashbeads2moretimeswith500µLicecolddilutionbuffer. 6.AddcelllysatetoequilibratedGFP-Trap®_AbeadsandincubatetheGFP-Trap®_Abeadswiththecelllysateunderconstantmixingfor10min–2hatroomtemperatureor4°C. note:duringincubationofproteinsamplewiththeGFP-Trap®_Athefinalconcentrationofdetergentsshouldnotexceed0.2%toavoidunspecificbindingtothematrix 7.Spintubeat2.500xgfor2minutesat4°C.Forwesternblotanalysisdilute50µLsupernatantwith50µL2xSDS-samplebuffer(àrefertoasnon-bound).Discardremainingsupernatant. 8.Washbeadsthreetimeswith500µLicecoldwashbuffer.Afterthelastwashstep,transferbeadstonewtube. optional:increasesaltconcentrationinthesecondwashingstepupto500mM 9.ResuspendGFP-Trap®_Abeadsin100µL2xSDS-Samplebufferorgotostep11. 10.Boilresuspendedbeadsfor10minutesat95°Ctodissociatetheimmunocomplexesfromthebeads.Thebeadscanbecollectedbycentrifugationat2.500xgfor2minutesat4°CandSDS-PAGEisperformedwiththesupernatant(àrefertoasbound). 11.optional:eluteboundproteinsbyadding50µL0.2MglycinepH2.5(incubationtime:30secunderconstantmixing)followedbycentrifugation.Transferthesupernatanttoafreshcupandadd5µL1MTrisbase(pH10.4)forneutralization.Toincreaseelutionefficiencythisstepcanberepeated.
Restrictions	ForResearchUseonly

Handling ReferencesImagesApplicationDetailsTargetdetailsbacktotop
Concentration	2.5mLresin
Buffer	20%EtOH
HandlingAdvice	Donotfreeze.
Storage	4°C
ExpiryDate	12months

Images ReferencesHandlingApplicationDetailsTargetdetailsbacktotop
Images ShowHide
SupplierImages	Left(IP):PulldownofGFPwithGFP-Trap®_AandGFP-Trap®_Mfrom293Tcellextracts.... ComparisonofGFP-Trapwithconventionalmono-andpolyclonalantibodies:Immunopreci... Imagesforproduct:GFP-Trap®A Left(IP):PulldownofGFPwithGFP-Trap®_AandGFP-Trap®_Mfrom293Tcellextracts.Input(I)andbound(B)fractionswereseparatedbySDS-PAGEfollowedbyCoomassiestaining.Right(Co-IP):PulldownofGFP-PCNAwithGFP-Trap®_AandGFP-Trap®_Mfrom293Tcellextracts.Otherbands:potentialinteractionpartnersofPCNA. ComparisonofGFP-Trapwithconventionalmono-andpolyclonalantibodies:Immunoprecipitations(IP)ofGFPfromproteinextractsofGFP-producinghumancells.Input(I),non-bound(FT)andbound(B)fractionswereseparatedbySDS-PAGEfollowedbyCoomassiestainingandWesternBlotting.(hc)heavychain,(lc)lightchainofconventionalantibodies.

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Productcitedin:	Braiterman,Gupta,Chaerkady,Cole,Hubbard:"CommunicationbetweentheN-andC-terminiisrequiredforCu-stimulatedSer/ThrphosphorylationofCu-(I)ATPase(ATP7B)."in:TheJournalofBIOLOGicalchemistry,2015(PubMed). Yan,Chu,Qin,Wang,Liu,Jin,Zhang,Gomez,Hergovich,Chen,He,Gao,Yao:"RegulationofNDR1activitybyPLK1ensuresproperspindleorientationinmitosis."in:Scientificreports,Vol.5,pp.10449,2015(PubMed). Xiao,MacNair,McGoldrick,McKeever,McLean,Zhang,Keith,Zinman,Rogaeva,Robertson:"IsoformSpecificAntibodiesRevealDistinctSubcellularLocalizationsofC9orf72inAmyotrophicLateralSclerosis."in:Annalsofneurology,2015(PubMed). Satpathy,Wagner,Beli,Gupta,Kristiansen,Malinova,Francavilla,Tolar,Bishop,Hostager,Choudhary:"Systems-wideanalysisofBCRsignalosomesanddownstreamphosphorylationandubiquitylation."in:Molecularsystemsbiology,Vol.11,Issue6,pp.810,2015(PubMed). Hyodo,Taniguchi,Manabe,Kaido,Mise,Sugawara,Taniguchi,Okuno:"PhosphatidicacidproducedbyphospholipaseDpromotesRNAreplicationofaplantRNAvirus. 品牌介绍 Antibodies online是抗体、ELISA试剂盒与其他相关试剂的在线营销商，提供来自250家著名供应商的1906775种研究试剂，涵盖广泛的研究应用。其中包括1391351种抗体，170000种ELISA试剂盒，197779种试剂盒，28984种二抗以及179554种蛋白质。公司介绍更多... Antibodies online是抗体、ELISA试剂盒与其他相关试剂的在线营销商，提供来自250家著名供应商的1906775种研究试剂，涵盖广泛的研究应用。其中包括1391351种抗体，170000种ELISA试剂盒，197779种试剂盒，28984种二抗以及179554种蛋白质。产品分类更多... Laboratory Accessories Plasma Buffers Other Accessory Reagents Proteins ELISA & Assay Kits Lysates, Serum, Plasma Antibody Pairs Tissues Primary Antibodies Arrays Activators Enzymes Secondary Antibodies Peptides Isotype & Other Controls Markers Beads and Resin Serum 销售排行更多... Antibodies-Online/Chicken whole blood (Non Sterile Sodium EDTA)/ABIN5026727/50 mL $166.32 Antibodies-Online/10K Spin Column/ABIN413915/25 each $235.00 Antibodies-Online/RFP-Trap® M/ABIN509412/20 tests $459.38 Antibodies-Online/CytoSelect™ Clonogenic Tumor Cell Isolation Kit (5 preps)/ABIN2344898/25 preparations $2854.50 Antibodies-Online/S100A8 antibody (S100 Calcium Binding Protein A8)/ABIN954654/0.2 mg $401.50 Antibodies-Online/Cation Channel, Sperm-Associated, gamma 2 (CATSPERG2) (AA 39-1063) protein (His tag)/ABIN3147239/0.5 m $7776.30 Antibodies-Online/Transcription Factor EB (TFEB) ELISA Kit/ABIN5526704/96 tests $503.80 Antibodies-Online（抗体在线）/AIF1 antibody (Allograft Inflammatory Factor 1) (AA 116-147)/100 μL/ABIN685477 $329.45 问答列表更多... Abcam一抗_Abcam抗体_Abcam化学试剂 In situ Hybridization on Tissue Sections A Method for... eurogentec PCR技术(十四):反向PCR PCR基础知识资讯血小板生长因子试剂盒血小板生长因子试剂盒批发厂家、厂家比价... Agrisera代理,Agrisera授权代理商,Agrisera总代理艾美捷科技有限... 抗生物素蛋白生物素复合物[过氧化物酶]技术的英文翻译_英语怎么说... mouse antigoat IgGPE Secondary Antibody \| SCBT 小肠结肠炎耶尔森氏菌(YE)核酸检测试剂盒(PCR荧光探针法)说明... Antibodiesonline公司 Antibodiesonline代理有限公... Array 厂家直采全球直采正品优价正品保障厂家直发有线跟踪正规清关 CIF100%正规报关，提供发票及时交付限时必达不达必赔快速导航：公司介绍产品中心新闻动态在线咨询联系我们公司介绍产品中心新闻动态在线咨询联系我们总部地址：江苏省苏州市工业园区星湖街218号苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125) 工作时间：周一至周五9:00~18:00 技术支持： Email：info@ebiomall.com 短信：18915418616 服务热线： 4000-520-616（全国） ☏ 0512-67156496（苏州）版权信息： © 2015- 苏州蚂蚁淘生物科技有限公司版权所有中国网站备案：苏ICP备17049038号-1 苏公网安备 32059002001627号友情链接：联系QQ:764052152 antibodiesinc代理商 atlanta公司 aviscera试剂抗体 beckman现货 aatbioquest促销 absoluteantibody accelagen targeting agrenvec alomone 免责声明：本站有部分内容来自互联网，如无意中侵犯了某个媒体、公司、企业或个人等的知识产权，请来电或致函告之，本网站将在规定时间内给予删除等相关处理。扫描二维码关注官方微信获取最新促销及产品信息

Handling ReferencesImagesApplicationDetailsTargetdetailsbacktotop
Handling ShowHide
Concentration	2.5mLresin
Buffer	20%EtOH
HandlingAdvice	Donotfreeze.
Storage	4°C
ExpiryDate	12months